Enzymes and Inhibitors
Thermo Scientific™ SfaAI (AsiSI) (10 U/μL)
Cut at GCGAT^CGC sites with SfaAI (AsiSI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsiSI, RgaI, SgfI).
Thermo Scientific™ NotI (10 U/μL)
The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI).
Thermo Scientific™ Eco130I (StyI) (10 U/μL)
Cut at C^CWWGG sites with Eco130I (StyI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI).
Thermo Scientific™ Kpn2I (BspEI) (10 U/μL)
The Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer (Isoschizomers: AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI).
Invitrogen™ Proteinase K, recombinant
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Thermo Scientific™ MauBI (5 U/μL)
Cut T CG^CGCGCG sites with MauBI restriction enzyme, which performs best at 37°C in Tango buffer.
Thermo Scientific™ NheI (10 U/μL)
The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).
Thermo Scientific™ PacI (10 U/μL)
The PacI restriction enzyme recognizes TTAAT^TAA sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ Pfl23II (BsiWI) (3 U/μL)
Cut at C^GTACG sites with Pfl23II (BsiWI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsiWI, PspLI).
Thermo Scientific™ MboI (10 U/μL)
The MboI restriction enzyme recognizes ^GATC sites and cuts best at 37°C in R buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm).
Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate
A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.
Invitrogen™ LR Clonase™ II Plus enzyme
Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.
Corning™ Dispase
Bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on BD Matrigel™ Basement Membrane Matrix
Thermo Scientific™ FastDigest BshTI
Cut at A^CCGGT sites with Thermo Scientific™ FastDigest™ AgeI (BshTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ Buffer R (10X)
Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer R, premixed with BSA for enhanced stability.