Enzymes and Inhibitors
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ NmuCI (Tsp45I) (10 U/μL)
Cut at ^GTSAC sites with NmuCI (Tsp45I) restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: Tsp45I).
Thermo Scientific™ FastDigest Eco130I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest FspAI
Cut at RTGC^GCAY sites with Thermo Scientific™ FastDigest™ FspAI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Invitrogen™ PI(3,4)P2 Lipid Kinase Substrate
The PI(3,4)P2 Lipid Kinase Substrate has been specifically optimized for use with Type I PIP kinases (e.g., PIP5K1A, PIP5K1B, PIP5K1C)
Thermo Scientific™ FastDigest Eco91I
Cut at G^GTNACC sites with Thermo Scientific™ FastDigest™ Eco91I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ AjuI (5 U/μL)
Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with AjuI restriction enzyme, which performs best at 37°C in R (+SAM) buffer.
Thermo Scientific™ FastDigest Esp3I (IIs class)
Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest Bsp1407I
Cut at T^GTACA sites with Bsp1407I restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: BsrGI, BstAUI).
Thermo Scientific™ FastDigest XagI
Cut at CCTNN^NNNAGG sites with Thermo Scientific™ FastDigest™ EcoNI (XagI), which performs best using universal FastDigest Buffer (Isoschizomers: BstENI).
Thermo Scientific™ LguI (SapI) (5 U/μL)
The LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: BspQI, PciSI, SapI).
Thermo Scientific™ FastDigest Bpu1102I
Cut at GC^TNAGC sites with Thermo Scientific™ FastDigest™ BlpI (Bpu1102I), which performs best using universal FastDigest Buffer (Isoschizomers: Bsp1720I, CelII).
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
Thermo Scientific™ Eco72I (PmlI) (10 U/μL)
Cut at CAC^GTG sites with Eco72I (PmlI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AcvI, BbrPI, PmaCI, PmlI, PspCI).