Enzymes and Inhibitors
Thermo Scientific™ KspAI (HpaI) (10 U/μL)
The KspAI (HpaI) restriction enzyme recognizes GTT^AAC sites and cuts best at 37°C in B buffer (Isoschizomers: HpaI).
Thermo Scientific™ TatI (5 U/μL)
Cut at W^GTACW sites with TatI restriction enzyme, which performs best at 65°C in Tango buffer.
Thermo Scientific™ BshNI (BanI) (10 U/μL)
Cut at G^GYRCC sites with BshNI (BanI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AccB1I, BanI, BspT107I).
Thermo Scientific™ PagI (BspHI) (10 U/μL)
The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).
Invitrogen™ SUMO Protease
A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae
Invitrogen™ DNase I, Amplification Grade
Purified and tested for nondetectable levels of RNase contamination
Invitrogen™ M13KO7 Helper Phage
Suitable for use with M13/phagemid hosts for the production of ssDNA from vectors containing an f1or f1-like origin of replication
Invitrogen™ X-Gal (5-Bromo-4-Chloro-3-Indolyl β-D-Galactopyranoside)
Widely used chromogenic substrate for β-galactosidase
Invitrogen™ CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide)
Cleavage produces blue-fluorescence
Invitrogen™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate)
Continuously assay acid phosphatases at low pH