Enzymes and Inhibitors
Invitrogen™ Anza™ 26 Eco32I
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ FastDigest Bpu1102I
Cut at GC^TNAGC sites with Thermo Scientific™ FastDigest™ BlpI (Bpu1102I), which performs best using universal FastDigest Buffer (Isoschizomers: Bsp1720I, CelII).
Thermo Scientific™ FastDigest Bsp119I
Cut at TT^CGAA sites with Thermo Scientific™ FastDigest™ Bsp119I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest AjuI
Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with Thermo Scientific™ FastDigest™ AjuI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ Eco52I (EagI) (10 U/μL)
The Eco52I (EagI) restriction enzyme recognizes C^GGCCG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BseX3I, BstZI, EagI, EclXI).
Thermo Scientific™ PagI (BspHI) (10 U/μL)
The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).
Thermo Scientific™ XapI (ApoI) (10 U/μL)
Cut at R^AATTY sites with XapI (ApoI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AcsI, ApoI).
Invitrogen™ Proteinase K, recombinant
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Thermo Scientific™ FastDigest XmiI
Cut at GT^MKAC sites with Thermo Scientific™ FastDigest™ AccI (XmiI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FblI).
Thermo Scientific™ TaiI (MaeII) (10 U/μL)
Cut at ACGT^ sites with TaiI (MaeII) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: HpyCH4IV, MaeII).
Thermo Scientific™ PacI (10 U/μL)
The PacI restriction enzyme recognizes TTAAT^TAA sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ BplI (5 U/μL)
Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer.
Thermo Scientific™ CpoI (RsrII) (10 U/μL)
The CpoI (RsrII) restriction enzyme recognizes CG^GWCCG sites and cuts best at 37°C in Tango buffer (Isoschizomers: CspI, Rsr2I, RsrII).